Genome Engineering Workshop

March 16 – 18, 2016

Utrecht University

Organizers: Mike Boxem (Utrecht University) and Mihail Sarov (MPI/CBG Dresden)

Registration deadline Jan. 21st!!

Scientific Programme

The goal of the workshop is to teach you how to design and execute genome engineering using CRISPR/Cas9. The focus will be on hands on training. We will provide a series of theoretical lectures, followed by hands on practice exercises and design of your own targets. You should therefore bring a laptop computer, and have a genome engineering target in mind. Topics that will be discussed include:

• Design of efficient sgRNAs
• Engineering of point mutations using single strand oligonucleotide repair templates
• Insertions of larger DNA fragments using plasmid-based repair templates
• Cloning of sgRNAs and repair templates
• Various co-CRISPR strategies

Preliminary Schedule

Wed March 16

2 pm – 5 pm Arrival and introductory lectures

Thu March 17

9:00 – 12:00 Lectures and practice exercises.

13:30 – 16:00 Design of your own engineering target

Fri March 18

9:00 – 12:00 Feedback, follow up on design problems, wrapup.

12:00 Lunch followed by departure

The schedule is not final and we will try to include actual practical demonstrations of injection procedures and techniques.

Venue

The workshop will be held at Utrecht University, The Netherlands. Utrecht is reached easily by a 30 min train ride from Schiphol International Airport. You will stay at the Malie Hotel, which is near the city center and a short 10 min bus ride from the University Campus.

Registration

There is a limit of 32 participants. To register, E-mail your COST username and contact info to m.boxem@uu.nl. If you do not have a COST username yet, create an account here.

This meeting is supported by COST action BM1408