Funded by the Horizon 2020 Framework Programme
of the European Union

LaserLab AMSTERDAM

Attached to Erwin Peterman Lab.

Advanced single-molecule fluorescence imaging equipment & image analysis tools, dedicated for C. elegans work.

Personne in charge : Erwin Peterman

Phone 0

LaserLab
De Boelelaan 1081,
1081HV, Amsterdam
Netherlands

LaserLab
De Boelelaan 1081,
1081HV, Amsterdam
Netherlands

Equipment description

apparatus-for-single-molecule-imaging

Super resolution microscopy
A592 nm orange CW laser (> 1W) for stimulated emission depletion (STED) microscopy connected to the Leica TCS SP5 II confocal microscope provides a two to threefold resolution improvement compared to standard confocal microscopy for several fluorescence channels.

Two photon microscopy
platform A tunable Mai Tai broadband laser (710-990 nm) connected to the inverted and the upright Leica TCS SP5 confocal microsopes, both with non descanned detectors. Excitation can be shared between the systems or used exclusively by one system.

Single Molecule Detection Platform (FLIM/FCS/FLCS)
A platform based on PicoQuant Hardware is connected to the inverted Leica SP5 confocal microscope for multiparametric single molecule detection and analysis. With it, Fluorescence Correlation Spectroscopy (FCS), Fluorescence Lifetime Imaging Microscopy (FLIM) and the combination of FCS with fluorescence lifetime, Fluorescence Lifetime Correlation Spectroscopy (FLCS) and gated FCS can be performed.
Additionally, the detectors can be used for the confocal imaging of weak signals